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Used as a "reporter" plasmid - which essentially means the luciferase (luciferin) plasmid (sm. circular piece of DNA) is inserted into a gene, the luciferin plasmid is then allowed to grow (incubate) inside the cells used for researching to determine whether a type of reaction has occurred or not occurred; albeit, it's considered a "reporter" or "trace" plasmid. As a series of Rxns occur in the process, the bioluminescence is an indication (a marker) of a particular outcome of the experiment. This is a common routine in conducting bioassays.
It has been used in many various types of bioassays both to elicit gene expression (when conducting simple/routine lab experiments to follow the course of one or several Rxns), as well as to follow the course of cellular propagation Rxns (e.g. validate diseased cell proliferation) & w/in select advanced cell sorting research via machines capable of selectively sorting cells from a consortia of other cells - primarily for data analysis. Most research facilities employ Sf9 cells derived from the mid-gut of the Army Worm Spodoptera frugiperda. Grow up a bucket of Sf9 cells, lyse the Sf9 cells, insert your gene of interest along w/ the luciferin plasmid, immerse the cells in a known substrate which oxidizes the luciferin (luciferase), isolate the proteins, genes, etc. of interest then analyze bioluminescence via several techniques. It isn't really witchcraft yet borderlines on trickery.
I used the same techniques described above but employed P32 &/or S35 radioisotopes in place of luciferase as a reporter/tracer/marker. More complicated but yields more definitive/exacting results.
Jim - You're in our prayers every day! Has there been any change on the possibility of you being a candidate for a transplant? I am sorry the dialysis is such a horrible burden on you!
Tanks, I got turned down on one program and in the process of trying another. The problem [reason was] I have some calcification in a major artery. I really should'nt complain. There s FB group named "I hate dialysis" Where peoples share horror stories about problems, The place is close and staffed with competent techs do very few screw up s/ Things could be alot worse
Been a pretty good morning so far but it seems slow. Maybe because it's Friday. Though we're working tomorrow so what does it matter.
Nice out but with the ever-constant threat of rain. No idea what to do after work.
Chris the trees like in Washington DC are what I want.
There is a beach in California that when you walk on it the pressure from your foot makes the sand glow green. Something about the algae in the water makes it do that. When you watch the surf come in you can see the glowing in the waves. I would call nature a miracle.
Think I'll sign a wheel or two. Hope everyone has a great day.
There is a beach in California that when you walk on it the pressure from your foot makes the sand glow green. Something about the algae in the water makes it do that.
Mike - It occurs all over the world. California is an example & the bioluminescence isn't restricted to beaches, it occurs in the open waters of every sea & ocean on the planet. The bioluminescence is caused by select bioluminescent dinoflagellates (which are a form of phytoplankton, i.e. algae). Not all dinoflaggelates are capable of bioluminescence (the majority are not). Roughly 90% of bioluminescent dinoflagellates occur in salt water environments; yet, they also occur in fresh water environments.
Tanks, I got turned down on one program and in the process of trying another. The problem [reason was] I have some calcification in a major artery. I really should'nt complain. There s FB group named "I hate dialysis" Where peoples share horror stories about problems, The place is close and staffed with competent techs do very few screw up s/ Things could be alot worse
Jim - You just go ahead and bitch to me/us, we don't mind, the whole dialysis thing sounds like a major PIA and just sucks for someone used to being very active like you. Good luck with the new program! 🙏
Probably some Japanese version. They were a gift to [someone] something like a hundred years ago from the Japanese prime minister. When I'm ready to plant them I'll find out and get some. Or later when I don't have any work to do.
Thanks Mike, lots of info there! Reading deeply into that info, I think what I may have is a "Weeping Cherry" because mine has the droopy branches and mult-colored (some white, some pink) blossoms. It also mentions many varieties (including Weeping Cherries) have incredible longevity living to be over 1000-yrs old! Wow!
Jim - You just go ahead and bitch to me/us, we don't mind, the whole dialysis thing sounds like a major PIA and just sucks for someone used to being very active like you. Good luck with the new program! 🙏
^^x2^^
Bitch, bitch, bitch all you care to bitch, bitch, bitch. Bothers me not in the slightest.
I have found some very unique and strange plants in our woods. Have you ever heard of the ghost plant aka indian pipe?
Art - Yes, I've seen/found them in several locations. I think the first time was in/around some Michigan U.P. peat bogs. Interesting eh? No chlorophyll like most plants yet they do feed on fungi.
Here's one more and then I won't bother you with anymore.
Conopholis americana (American squaw-root)
Art - I have seen the Ghost Pipe in both Michigan where I grew up and here in Missouri, I think it's range is pretty large. I have seen the Squaw Root in Michigan, but not here in Missouri. I usually saw the Squaw Root growing on/into the exposed roots of trees if I'm remembering correctly? I spent alot of time outdoors hunting, fishing, camping, canoeing growing up in Michigan, if it's out there up there, I probably saw it at one time or another.
Not sure I've ever seen or recognized a Squaw Root.
Here's one I've seen on numerous occasions only because I knew what I was looking for...you gotta be nearly knee deep in the quagmire to find this one.
Not sure I've ever seen or recognized a Squaw Root.
Here's one I've seen on numerous occasions only because I knew what I was looking for...you gotta be nearly knee deep in the quagmire to find this one.
I had never seen the Squaw Root before and I had a hard time finding out any information about it. What I did learn is it's very rare to find them in my state. What's fascinating about them is they are a plant species by them selves. They are neither a mushroom nor a lichen or a typical plant. They feed off of fungus but they do not give anything back, sort of a leech.
I wonder if when Maggie wakes up that her tongue falls to sleep from laying on it like that?
I wonder if when Maggie wakes up that her tongue falls to sleep from laying on it like that?
Art - That is a good question that I don't have a good answer for? What I do know is that both of our Bulldogs seem to have tongues that are way too large for their smushed in faces, and they are hanging out to some degree most of the time, awake or asleep.
Art - That is a good question that I don't have a good answer for? What I do know is that both of our Bulldogs seem to have tongues that are way too large for their smushed in faces, and they are hanging out to some degree most of the time, awake or asleep.
They're both cuties no matter what. I smile whenever I see a pic of them. I hope they're doing better now after the surgeries.
Very good article and information.
I saw a video on youtube of some squirrels that had been eating some mushrooms that were growing in the grass, it was really funny watching them stoned out of their minds.
I'm on Cloud Nine when I find a patch of my favorite woodland spp. Oh hell yes. Lightly breaded sauteed in butter - OMG!
Norm - I absolutely love Morels cooked just like you, can't beat em! Used to go hunting for them with my Dad when I was young and we would usually find our fair share. I have never been out here, but my SIL Justin, Gabe, and Keegan go out looking around their place and have had good luck. They have a huge north facing hillside directly behind their house that has very tall old growth timber on it with not much undergrowth and they find quite a few there.
They're both cuties no matter what. I smile whenever I see a pic of them. I hope they're doing better now after the surgeries.
Thanks Art, I really appreciate your kind comments. They are both doing exceptionally well since their surgeries, and we are over the moon with the results! A tremendous quality of life improvement for both of them!
I missed looking for morels on the new place this year. Just too damn busy.
I am glad the dogs can breathe. I think we've done too much on dog varieties some times. If a dog can't run, and can't swim, or can't breathe, should we really have played God to make that breed that way? The study of dogs is fascinating to see what was done millennia ago to domesticate them for various purposes. My mother has a beagle. It is a pretty, good natured dog that will do anything for food, but it is stubborn and easily distracted. My mother gardens and the beagle watches a rabbit with intensity when it shows up. I have to remind her that rabbits are literally the reason for the beagle; they are rabbit dogs. The English Bulldog is even more interesting, as it seems that it was bred from the Olde English Bulldog, which was actually used to bait bulls until that sport was banned. I think most traditional breeds had a job, unlike the labradoodle weird things of today.
I was going to go to the Kentucky plant on Tues night, but my laptop needed work, so I went Wed night. Taught a class all day Thurs at headquarters. Got good BBQ for lunch comped. Finished up, visited my friend for some parts in the plant proper, and went and got dinner then to bed. Slept in today some at hotel, got going, drove partially back to kill some time, went shopping at bit at the outlet mall outside Louisville. Had a staff meeting online, then I went back a couple miles to a lawn care place and put down stupid money on a Ventrac tractor and two attachments. They were happy for the sale and will deliver. I bought a 4520 tractor with the better Kubota gas engine and the pro package, along with a bunch of other upgrades. My thoughts were to get an upgraded tractor now, and buy more attachments later as needed. I bought a wide finish deck and a "tough cut" deck which seems to be aimed at overgrowth and fields.
There are currently 35 attachments available. I am buying 2 now, I could foresee the need for 10 more, but, in reality, I will probably only buy 4 or so others in a couple years. I want a plow for snow, perhaps a tiller for a garden, some sort of bucket, and maybe an edger or the super brush bush hawg thing. They'll deliver this tractor and two things to me shortly, and that's about all the bank account can take for this year.
I may go mow with the old mowers tomorrow depending on how dry it is.
